a Record control number 000253323400012

a ISSN 1088-5412

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number WD300

b Item number .T74 2008

a Personal name Yazi, Didem.

h Medium Electronic Document

a Title Treatment with Mycobacterium vaccae ameliorates airway histopathology in a murine model of asthma.

c Statement of responsibility, etc Didem Yazi, Tunc Akkoc, Ozlem Yesil, Cevdet Ozdernir, Metin Aydogan, Kaya Koksalan, Nerin N. Bahceciler, Isil B. Barlan.

b Name of publisher, distributor, etc Ocean Side Publications Inc.,

c Date of publication, distribution, etc 2008.

a Summary, etc The objective of this study was to evaluate the effect of intratracheal (i.t.) or subcutaneous (s.c.) Mycobacterium vaccae treatment on lung histopathology and cytokine responses in a murine model of asthma. BALB/c mice were divided into four groups. To establish an asthma model, Groups I, II and III received intraperitoneal (i.p.) ovalbumin (OVA) and were challenged with i.t. OVA three times (days 41-47). On the same days, mice in Groups I and II were treated with i.t. and s.c. Mycobacterium vaccae, respectively. Mice in Group IV served as controls. On day 49, lungs were taken out for histopathological evaluation. Cytokine levels were determined in splenocyte culture supernatants by ELISA. The thickness of basement membrane and hyperplasic goblet cells in small airways were found to be significantly more in Group III than Group I. Furthermore, smooth muscle and epithelial thickness in small and large airways and hyperplasic goblet cell numbers in all sized airways of this treatment group were not significantly different from controls. Epithelial thickness in medium and large airways, hyperplasic goblet cells in all sized airways, and basement membrane in small and large airways were not significantly different in Group II when compared to controls. OVA-stimulated IL-5 levels was significantly higher in Group I when compared to Group III. OVA-stimulated IL-5 and spontaneous IL-5 levels were significantly higher in Group 11 than Group III. We demonstrate that subcutaneous and intratracheal Mycobacterium vaccae administered along with allergen has an ameliorating effect in the modulation of airway histopathological changes in OVA sensitized mice.

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Allergy

a Topical term or geographic name as entry element Airway histopathology

a Topical term or geographic name as entry element Asthma

g Relationship information JAN-FEB 2008, Vol. 29 Issue 1, p67-73

t Title Pediatric Allergy And Immunology

x International Standard Serial Number 10885412

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.2500/aap2008.29.3082